murine sfrp1 Search Results


murine sfrp1  (Addgene inc)
93
Addgene inc murine sfrp1
Wnt inhibition results in altered dendritic spine development. (A) Representative dendritic segments of cortical neurons expressing empty vector (EV), Wif1, <t>Sfrp1,</t> Fzd8CRD or Dvl1ΔPDZ. (B) Quantification of dendritic protrusion density. (C) Percent of all dendritic protrusions classified as either spines or filopodia. Quantification of average protrusion length (D) and average spine head width (E) for each treatment. *** p < 0.001, ** p < 0.01, * p < 0.05. n = number of neurons: EV n = 31, Wif1 n = 34 Sfrp1 n = 23, Fzd8CRD n = 25, Dvl1ΔPDZ n = 25. Scale bar: 5 μm.
Murine Sfrp1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/murine+sfrp1/pRK5+mFz8CRD-IgG+(Plasmid+%2316689)/pmc03793870-174-45-51
Average 93 stars, based on 1 article reviews
murine sfrp1 - by Bioz Stars, 2026-10
93/100 stars
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recombinant murine sfrp1  (R&D Systems)
93
R&D Systems recombinant murine sfrp1
Wnt inhibition results in altered dendritic spine development. (A) Representative dendritic segments of cortical neurons expressing empty vector (EV), Wif1, <t>Sfrp1,</t> Fzd8CRD or Dvl1ΔPDZ. (B) Quantification of dendritic protrusion density. (C) Percent of all dendritic protrusions classified as either spines or filopodia. Quantification of average protrusion length (D) and average spine head width (E) for each treatment. *** p < 0.001, ** p < 0.01, * p < 0.05. n = number of neurons: EV n = 31, Wif1 n = 34 Sfrp1 n = 23, Fzd8CRD n = 25, Dvl1ΔPDZ n = 25. Scale bar: 5 μm.
Recombinant Murine Sfrp1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/murine+sfrp1/Recombinant+Human+sFRP-1+Protein%2C+CF/pm22040599-41-0-14
Average 93 stars, based on 1 article reviews
recombinant murine sfrp1 - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier

Image Search Results


Wnt inhibition results in altered dendritic spine development. (A) Representative dendritic segments of cortical neurons expressing empty vector (EV), Wif1, Sfrp1, Fzd8CRD or Dvl1ΔPDZ. (B) Quantification of dendritic protrusion density. (C) Percent of all dendritic protrusions classified as either spines or filopodia. Quantification of average protrusion length (D) and average spine head width (E) for each treatment. *** p < 0.001, ** p < 0.01, * p < 0.05. n = number of neurons: EV n = 31, Wif1 n = 34 Sfrp1 n = 23, Fzd8CRD n = 25, Dvl1ΔPDZ n = 25. Scale bar: 5 μm.

Journal: Molecular and Cellular Neurosciences

Article Title: Neurotrophin and Wnt signaling cooperatively regulate dendritic spine formation ☆

doi: 10.1016/j.mcn.2013.04.006

Figure Lengend Snippet: Wnt inhibition results in altered dendritic spine development. (A) Representative dendritic segments of cortical neurons expressing empty vector (EV), Wif1, Sfrp1, Fzd8CRD or Dvl1ΔPDZ. (B) Quantification of dendritic protrusion density. (C) Percent of all dendritic protrusions classified as either spines or filopodia. Quantification of average protrusion length (D) and average spine head width (E) for each treatment. *** p < 0.001, ** p < 0.01, * p < 0.05. n = number of neurons: EV n = 31, Wif1 n = 34 Sfrp1 n = 23, Fzd8CRD n = 25, Dvl1ΔPDZ n = 25. Scale bar: 5 μm.

Article Snippet: pCMV-BDNF was constructed by inserting the open reading frame for murine BDNF into the pEGFP-N1 backbone (Clontech, Mountain View, CA). pBR22-Wif1 expresses murine Wif1 and was a generous gift from Dr. Ken Iwatsuki (Ajinomoto Co., Tokyo, Japan). pCS2 +-Sfrp1 (Addgene plasmid 16693, Cambridge, MA) expresses murine Sfrp1 (Randall Moon Lab). pRK5-mFzd8CRD-IgG (Addgene plasmid 16689) expresses a fusion protein consisting of the extracellular domain of the murine Frizzled-8 protein fused with the human immunoglobulin heavy chain ( ). pCS2 +-mDvl1ΔPDZ-HA expresses a C-terminally HA tagged murine Dishevelled-1 with a deletion of amino acids 276–336 ( ). pTRE-tight-BDNF and pTRE-tight-Wnt2 were constructed by inserting the ORFs for murine BDNF and murine Wnt2, respectively, into the pTRE-tight backbone (Clontech). pTRE-tight-EGFP was constructed by inserting the EGFP ORF from pEGFP-N1 (Clontech) into the pTRE-tight backbone. pCMV-rtTA was constructed by inserting the ORF for the reverse-tet-Transactivator protein from pTRIPZ (Thermo Scientific) into the pEGFP-N1 backbone from which the EGFP coding region was deleted.

Techniques: Inhibition, Expressing, Plasmid Preparation

Expression of Wnt inhibitors or Wnt2 shifts the distribution of dendritic protrusion lengths. (A) Relative frequency distributions of dendritic protrusion length comparing Wif1, Sfrp1, Fzd8CRD and Dvl1ΔPDZ expressing neurons to EV control. (B) Relative frequency distribution of protrusion length comparing Wnt2 expressing neurons to EV control. n = number of neurons: EV(A) n = 31, Wif1 n = 34, Sfrp1 n = 23, Fzd8CRD n = 25, Dvl1ΔPDZ n = 25; EV(B) n = 29, Wnt2 n = 25. *** p < 0.001, ** p < 0.01, * p < 0.05.

Journal: Molecular and Cellular Neurosciences

Article Title: Neurotrophin and Wnt signaling cooperatively regulate dendritic spine formation ☆

doi: 10.1016/j.mcn.2013.04.006

Figure Lengend Snippet: Expression of Wnt inhibitors or Wnt2 shifts the distribution of dendritic protrusion lengths. (A) Relative frequency distributions of dendritic protrusion length comparing Wif1, Sfrp1, Fzd8CRD and Dvl1ΔPDZ expressing neurons to EV control. (B) Relative frequency distribution of protrusion length comparing Wnt2 expressing neurons to EV control. n = number of neurons: EV(A) n = 31, Wif1 n = 34, Sfrp1 n = 23, Fzd8CRD n = 25, Dvl1ΔPDZ n = 25; EV(B) n = 29, Wnt2 n = 25. *** p < 0.001, ** p < 0.01, * p < 0.05.

Article Snippet: pCMV-BDNF was constructed by inserting the open reading frame for murine BDNF into the pEGFP-N1 backbone (Clontech, Mountain View, CA). pBR22-Wif1 expresses murine Wif1 and was a generous gift from Dr. Ken Iwatsuki (Ajinomoto Co., Tokyo, Japan). pCS2 +-Sfrp1 (Addgene plasmid 16693, Cambridge, MA) expresses murine Sfrp1 (Randall Moon Lab). pRK5-mFzd8CRD-IgG (Addgene plasmid 16689) expresses a fusion protein consisting of the extracellular domain of the murine Frizzled-8 protein fused with the human immunoglobulin heavy chain ( ). pCS2 +-mDvl1ΔPDZ-HA expresses a C-terminally HA tagged murine Dishevelled-1 with a deletion of amino acids 276–336 ( ). pTRE-tight-BDNF and pTRE-tight-Wnt2 were constructed by inserting the ORFs for murine BDNF and murine Wnt2, respectively, into the pTRE-tight backbone (Clontech). pTRE-tight-EGFP was constructed by inserting the EGFP ORF from pEGFP-N1 (Clontech) into the pTRE-tight backbone. pCMV-rtTA was constructed by inserting the ORF for the reverse-tet-Transactivator protein from pTRIPZ (Thermo Scientific) into the pEGFP-N1 backbone from which the EGFP coding region was deleted.

Techniques: Expressing, Control

Wnt inhibition results in decreased dendrite elaboration. (A) Representative cortical neurons expressing EV, Wif1, Sfrp1, Fzd8CRD or Dvl1ΔPDZ. (B) Quantification of the total dendrite length per neuron for each treatment. (C) Quantification of the number of dendritic endpoints per neuron for each treatment. (D–G) Sholl analysis of dendritic complexity comparing neurons treated with each Wnt inhibitor to control neurons. *** p < 0.001, ** p < 0.01, * p < 0.05. n = number of neurons: EV n = 56, Wif1 n = 49, Sfrp1 n = 39, Fzd8CRD n = 39, Dvl1ΔPDZ n = 39. Scale bar: 50 μm.

Journal: Molecular and Cellular Neurosciences

Article Title: Neurotrophin and Wnt signaling cooperatively regulate dendritic spine formation ☆

doi: 10.1016/j.mcn.2013.04.006

Figure Lengend Snippet: Wnt inhibition results in decreased dendrite elaboration. (A) Representative cortical neurons expressing EV, Wif1, Sfrp1, Fzd8CRD or Dvl1ΔPDZ. (B) Quantification of the total dendrite length per neuron for each treatment. (C) Quantification of the number of dendritic endpoints per neuron for each treatment. (D–G) Sholl analysis of dendritic complexity comparing neurons treated with each Wnt inhibitor to control neurons. *** p < 0.001, ** p < 0.01, * p < 0.05. n = number of neurons: EV n = 56, Wif1 n = 49, Sfrp1 n = 39, Fzd8CRD n = 39, Dvl1ΔPDZ n = 39. Scale bar: 50 μm.

Article Snippet: pCMV-BDNF was constructed by inserting the open reading frame for murine BDNF into the pEGFP-N1 backbone (Clontech, Mountain View, CA). pBR22-Wif1 expresses murine Wif1 and was a generous gift from Dr. Ken Iwatsuki (Ajinomoto Co., Tokyo, Japan). pCS2 +-Sfrp1 (Addgene plasmid 16693, Cambridge, MA) expresses murine Sfrp1 (Randall Moon Lab). pRK5-mFzd8CRD-IgG (Addgene plasmid 16689) expresses a fusion protein consisting of the extracellular domain of the murine Frizzled-8 protein fused with the human immunoglobulin heavy chain ( ). pCS2 +-mDvl1ΔPDZ-HA expresses a C-terminally HA tagged murine Dishevelled-1 with a deletion of amino acids 276–336 ( ). pTRE-tight-BDNF and pTRE-tight-Wnt2 were constructed by inserting the ORFs for murine BDNF and murine Wnt2, respectively, into the pTRE-tight backbone (Clontech). pTRE-tight-EGFP was constructed by inserting the EGFP ORF from pEGFP-N1 (Clontech) into the pTRE-tight backbone. pCMV-rtTA was constructed by inserting the ORF for the reverse-tet-Transactivator protein from pTRIPZ (Thermo Scientific) into the pEGFP-N1 backbone from which the EGFP coding region was deleted.

Techniques: Inhibition, Expressing, Control

Wnt inhibition does not affect primary dendrite number on cortical neurons. (A) Representative cell bodies of cortical neurons expressing EV, Wif1, Sfrp1, Fzd8CRD and Dvl1ΔPDZ either alone, or in combination with BDNF. (B) Quantification of the number of primary dendrites per neuron for each treatment. *** p < 0.001. n = number of neurons: EV n = 31, BDNF n = 31, Wif1 n = 34, BDNF + Wif1 n = 29, Sfrp1 n = 22, BDNF + Sfrp1 n = 21, Fzd8CRD n = 25, BDNF + Fzd8CRD n = 25, Dvl1ΔPDZ n = 25, BDNF + Dvl1ΔPDZ n = 30. Scale bar: 5 μm.

Journal: Molecular and Cellular Neurosciences

Article Title: Neurotrophin and Wnt signaling cooperatively regulate dendritic spine formation ☆

doi: 10.1016/j.mcn.2013.04.006

Figure Lengend Snippet: Wnt inhibition does not affect primary dendrite number on cortical neurons. (A) Representative cell bodies of cortical neurons expressing EV, Wif1, Sfrp1, Fzd8CRD and Dvl1ΔPDZ either alone, or in combination with BDNF. (B) Quantification of the number of primary dendrites per neuron for each treatment. *** p < 0.001. n = number of neurons: EV n = 31, BDNF n = 31, Wif1 n = 34, BDNF + Wif1 n = 29, Sfrp1 n = 22, BDNF + Sfrp1 n = 21, Fzd8CRD n = 25, BDNF + Fzd8CRD n = 25, Dvl1ΔPDZ n = 25, BDNF + Dvl1ΔPDZ n = 30. Scale bar: 5 μm.

Article Snippet: pCMV-BDNF was constructed by inserting the open reading frame for murine BDNF into the pEGFP-N1 backbone (Clontech, Mountain View, CA). pBR22-Wif1 expresses murine Wif1 and was a generous gift from Dr. Ken Iwatsuki (Ajinomoto Co., Tokyo, Japan). pCS2 +-Sfrp1 (Addgene plasmid 16693, Cambridge, MA) expresses murine Sfrp1 (Randall Moon Lab). pRK5-mFzd8CRD-IgG (Addgene plasmid 16689) expresses a fusion protein consisting of the extracellular domain of the murine Frizzled-8 protein fused with the human immunoglobulin heavy chain ( ). pCS2 +-mDvl1ΔPDZ-HA expresses a C-terminally HA tagged murine Dishevelled-1 with a deletion of amino acids 276–336 ( ). pTRE-tight-BDNF and pTRE-tight-Wnt2 were constructed by inserting the ORFs for murine BDNF and murine Wnt2, respectively, into the pTRE-tight backbone (Clontech). pTRE-tight-EGFP was constructed by inserting the EGFP ORF from pEGFP-N1 (Clontech) into the pTRE-tight backbone. pCMV-rtTA was constructed by inserting the ORF for the reverse-tet-Transactivator protein from pTRIPZ (Thermo Scientific) into the pEGFP-N1 backbone from which the EGFP coding region was deleted.

Techniques: Inhibition, Expressing

Wnt inhibitors interfere with BDNF-induced dendritic spine formation. (A) Representative dendritic segments of cortical neurons expressing EV, BDNF with EV, BDNF with Wif1, BDNF with Sfrp1, BDNF with Fzd8CRD and BDNF with Dvl1ΔPDZ. (B) Quantification of dendritic protrusion density. (C) Fraction of all spines classified as either spines or filopodia. Quantification of average protrusion length (D) and average spine head width (E) for each treatment. *** p < 0.001, ** p < 0.01, * p < 0.5. (* = relative to EV, # = relative to EV + BDNF.) n = number of neurons: EV n = 31, BDNF + EV n = 30, BDNF + Wif1 n = 29, BDNF + Sfrp1 n = 25, BDNF + Fzd8CRD n = 25, BDNF + Dvl1ΔPDZ n = 30. Scale bar: 5 μm.

Journal: Molecular and Cellular Neurosciences

Article Title: Neurotrophin and Wnt signaling cooperatively regulate dendritic spine formation ☆

doi: 10.1016/j.mcn.2013.04.006

Figure Lengend Snippet: Wnt inhibitors interfere with BDNF-induced dendritic spine formation. (A) Representative dendritic segments of cortical neurons expressing EV, BDNF with EV, BDNF with Wif1, BDNF with Sfrp1, BDNF with Fzd8CRD and BDNF with Dvl1ΔPDZ. (B) Quantification of dendritic protrusion density. (C) Fraction of all spines classified as either spines or filopodia. Quantification of average protrusion length (D) and average spine head width (E) for each treatment. *** p < 0.001, ** p < 0.01, * p < 0.5. (* = relative to EV, # = relative to EV + BDNF.) n = number of neurons: EV n = 31, BDNF + EV n = 30, BDNF + Wif1 n = 29, BDNF + Sfrp1 n = 25, BDNF + Fzd8CRD n = 25, BDNF + Dvl1ΔPDZ n = 30. Scale bar: 5 μm.

Article Snippet: pCMV-BDNF was constructed by inserting the open reading frame for murine BDNF into the pEGFP-N1 backbone (Clontech, Mountain View, CA). pBR22-Wif1 expresses murine Wif1 and was a generous gift from Dr. Ken Iwatsuki (Ajinomoto Co., Tokyo, Japan). pCS2 +-Sfrp1 (Addgene plasmid 16693, Cambridge, MA) expresses murine Sfrp1 (Randall Moon Lab). pRK5-mFzd8CRD-IgG (Addgene plasmid 16689) expresses a fusion protein consisting of the extracellular domain of the murine Frizzled-8 protein fused with the human immunoglobulin heavy chain ( ). pCS2 +-mDvl1ΔPDZ-HA expresses a C-terminally HA tagged murine Dishevelled-1 with a deletion of amino acids 276–336 ( ). pTRE-tight-BDNF and pTRE-tight-Wnt2 were constructed by inserting the ORFs for murine BDNF and murine Wnt2, respectively, into the pTRE-tight backbone (Clontech). pTRE-tight-EGFP was constructed by inserting the EGFP ORF from pEGFP-N1 (Clontech) into the pTRE-tight backbone. pCMV-rtTA was constructed by inserting the ORF for the reverse-tet-Transactivator protein from pTRIPZ (Thermo Scientific) into the pEGFP-N1 backbone from which the EGFP coding region was deleted.

Techniques: Expressing

Transfection with Wnt inhibitors does not prevent BDNF-induced c-Fos expression in cortical neurons. (A) GFP fluorescence, c-Fos immunoreactivity and DAPI fluorescence of representative cell bodies of cortical neurons expressing EV, Wif1, Sfrp1, Fzd8CRD and Dvl1ΔPDZ either alone, or in combination with BDNF. (B) Quantification of normalized nuclear c-Fos immunoreactivity per neuron for each treatment. *** p < 0.001. n = 10 neurons for each treatment.

Journal: Molecular and Cellular Neurosciences

Article Title: Neurotrophin and Wnt signaling cooperatively regulate dendritic spine formation ☆

doi: 10.1016/j.mcn.2013.04.006

Figure Lengend Snippet: Transfection with Wnt inhibitors does not prevent BDNF-induced c-Fos expression in cortical neurons. (A) GFP fluorescence, c-Fos immunoreactivity and DAPI fluorescence of representative cell bodies of cortical neurons expressing EV, Wif1, Sfrp1, Fzd8CRD and Dvl1ΔPDZ either alone, or in combination with BDNF. (B) Quantification of normalized nuclear c-Fos immunoreactivity per neuron for each treatment. *** p < 0.001. n = 10 neurons for each treatment.

Article Snippet: pCMV-BDNF was constructed by inserting the open reading frame for murine BDNF into the pEGFP-N1 backbone (Clontech, Mountain View, CA). pBR22-Wif1 expresses murine Wif1 and was a generous gift from Dr. Ken Iwatsuki (Ajinomoto Co., Tokyo, Japan). pCS2 +-Sfrp1 (Addgene plasmid 16693, Cambridge, MA) expresses murine Sfrp1 (Randall Moon Lab). pRK5-mFzd8CRD-IgG (Addgene plasmid 16689) expresses a fusion protein consisting of the extracellular domain of the murine Frizzled-8 protein fused with the human immunoglobulin heavy chain ( ). pCS2 +-mDvl1ΔPDZ-HA expresses a C-terminally HA tagged murine Dishevelled-1 with a deletion of amino acids 276–336 ( ). pTRE-tight-BDNF and pTRE-tight-Wnt2 were constructed by inserting the ORFs for murine BDNF and murine Wnt2, respectively, into the pTRE-tight backbone (Clontech). pTRE-tight-EGFP was constructed by inserting the EGFP ORF from pEGFP-N1 (Clontech) into the pTRE-tight backbone. pCMV-rtTA was constructed by inserting the ORF for the reverse-tet-Transactivator protein from pTRIPZ (Thermo Scientific) into the pEGFP-N1 backbone from which the EGFP coding region was deleted.

Techniques: Transfection, Expressing, Fluorescence